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KMID : 0358219950220020109
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Korean Journal of Fertility and Sterility
1995 Volume.22 No. 2 p.109 ~ p.122
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High Resolution G- and R-banding for Acurate analysis in Human Chromosomes
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Abstract
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A technique is described for producing high resolution G-and R-banded chromosomes in human peripheral lymphocyte cultures. Cultured lymphocyte cells exposed to ethidium bromide (10μg/ml) and colcemid (0.02μg/ml) each for 2.5h and 0.5h prior to
harvest
for high resolution G-banded chromosomes. High resolution R-band patterns were obtained by BrdU substitution which was revealed by the fluorochrome-photolysis-Giemsa staining technique.
These methods are easy to perform and highly reliable. The data on relative length of chromosomes at the four mitotic stages are presented in units of percentage of haploid autosome length. The characteristic patterns of GTG-bands after trypsin
and
Giemsa) and RBG bands (R-bands after BrdU and Giemsa) were analyzed.
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KEYWORD
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